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smart-seq2 method  (Illumina Inc)


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    Structured Review

    Illumina Inc smart-seq2 method
    Smart Seq2 Method, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smart+seq2+method/smart+seq2+libraries/med_rxiv__2023__02__10__23285332-59-6-12
    Average 90 stars, based on 1 article reviews
    smart-seq2 method - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    RNA sequencing:

    Article Title: Transcriptional profiling of macrophages reveals distinct parasite stage-driven signatures during early infection by Leishmania donovani
    Article Snippet: Purity and integrity of RNA was assessed using a Bioanalyzer 2100 with an Eukaryote Total RNA Nano chip (Agilent Technologies). .. RNAseq libraries were generated using the Smart-seq2 method and sequenced by using an Illumina HiSeq2500 instrument with a single-end 51-base sequencing setup from three independent biological replicates for uninfected and L. donovani promastigote-infected BMDMs, and five independent biological replicates for L. donovani amastigote-infected BMDMs. ..

    Generated:

    Article Title: Transcriptional profiling of macrophages reveals distinct parasite stage-driven signatures during early infection by Leishmania donovani
    Article Snippet: Purity and integrity of RNA was assessed using a Bioanalyzer 2100 with an Eukaryote Total RNA Nano chip (Agilent Technologies). .. RNAseq libraries were generated using the Smart-seq2 method and sequenced by using an Illumina HiSeq2500 instrument with a single-end 51-base sequencing setup from three independent biological replicates for uninfected and L. donovani promastigote-infected BMDMs, and five independent biological replicates for L. donovani amastigote-infected BMDMs. ..

    Sequencing:

    Article Title: Transcriptional profiling of macrophages reveals distinct parasite stage-driven signatures during early infection by Leishmania donovani
    Article Snippet: Purity and integrity of RNA was assessed using a Bioanalyzer 2100 with an Eukaryote Total RNA Nano chip (Agilent Technologies). .. RNAseq libraries were generated using the Smart-seq2 method and sequenced by using an Illumina HiSeq2500 instrument with a single-end 51-base sequencing setup from three independent biological replicates for uninfected and L. donovani promastigote-infected BMDMs, and five independent biological replicates for L. donovani amastigote-infected BMDMs. ..

    RNA Sequencing:

    Article Title: Sustained systemic hyaluronan in COVID-19 patients, a 3D-lung model reveals mechanisms of overproduction counteracted by cortisone
    Article Snippet: The HBEC ALI-cultures were then lysed and RNA extraction was done using the NucleoSpin RNA II kit (Macherey-Nagel) following the manufacturer’s instructions. .. RNA-seq libraries were prepared using the Smart-seq2 method, and sequenced on an Illumina NextSeq 500 (75PE, v2.5 High Output kit). ..

    Article Title: HUMAN PLASMA TRANSCRIPTOME ANALYSIS IN BRAIN-ENRICHED EXTRACELLULAR VESICLES WITH BIPOLAR DISORDER AND MAJOR DEPRESSION
    Article Snippet: We purified total RNA from NCAM-1 positive EVs using RNeasy micro kit (QIAGEN). .. RNA-seq library was prepared using Smart-seq2 method and Nextera XT DNA Library Preparation Kit (Illumina). ..

    DNA Library Preparation:

    Article Title: HUMAN PLASMA TRANSCRIPTOME ANALYSIS IN BRAIN-ENRICHED EXTRACELLULAR VESICLES WITH BIPOLAR DISORDER AND MAJOR DEPRESSION
    Article Snippet: We purified total RNA from NCAM-1 positive EVs using RNeasy micro kit (QIAGEN). .. RNA-seq library was prepared using Smart-seq2 method and Nextera XT DNA Library Preparation Kit (Illumina). ..

    other:

    Article Title: Depletion of Hemoglobin Transcripts and Long-Read Sequencing Improves the Transcriptome Annotation of the Polar Bear ( Ursus maritimus )
    Article Snippet: As a proof of concept, we evaluated three hemoglobin-depleted and non-depleted polar bear whole blood transcriptomes using our ONT-based R2C2 ( ) full-length cDNA sequencing method and an Illumina-based modified Smart-seq2 method.



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